Epiphytic bet-mannanase producing bacterial strains

Dry remains of the herbal species plantain ( Plantago major ), wormwood Artemisia vulgaris ) and reed grass Calamagrostis acutiflora were used as a natural source for isolation β- mannanase producing strains. They isolated by using carob gum single carbon energy. Each chosen plant was found to be colonized with dominant epiphytic group microorganism, although plants had been collected in same location. Bacillus circulans only P. , subtilis on A. whereas Pantoea sp. C. . Identification taxonomy affiliation β-mannanase producers allowed formerly proposed primers PCR cloning genes previously occurred respective bacterial species. This approach let us 330 bp fragment from B. 1000 gene Testing enzymatic activity strains staining hydrolysis zones plates Congo Red carried out. As result, maximum